Avaliação dos efeitos do complexo B no processo de cicatrização de tecido mole em ratos Wistar / Evaluation of the effects of B complex on the healing process of Wistar rat soft tissue

Estudos mostram que as vitaminas do complexo B podem ser importantes no processo de cura e reparo. Considerando a escassez de estudos que avaliam o papel das vitaminas do complexo B no processo de cicatrização de feridas cirúrgicas e seu potencial uso em Odontologia, o objetivo deste estudo foi avaliar os efeitos do complexo B, aplicado por via tópica no processo de cicatrização de tecidos moles em ratos Wistar, além de comparar com o tratamento a laser. As feridas foram produzidas em 60 ratos Wistar. Duas lesões foram induzidas em cada rato, um com região dorsal e outro no palato duro, usando bisturi cirúrgico circular. Os animais foram divididos em 3 grupos: Grupo 1 (controle negativo) - tratado com 0,9% de solução fisiológica; Grupo 2 (controle positivo) - tratado com laser e Grupo 3 (experimental) ­ tratado com as vitaminas do complexo B. Os grupos receberam tratamentos diários durante 14 dias. Os diâmetros das lesões foram medidos a cada três dias. A cicatrização das lesões ocorreu em 14 dias, mostrando que a evolução dos animais tratados com as vitaminas do complexo B foi melhor durante o tratamento quando comparado ao grupo controle, podendo sugerir que as vitaminas do complexo B influenciam o processo de cicatrização de tecidos moles (AU).

Some previous studies have shown that the B complex vitamins may be important in the healing and repair process. Considering the scarcity of studies that evaluate the role of vitamin B complex in the process of surgical wound healing and its potential use in Dentistry, the aim of this study was to evaluate the effects of the B complex applied by topical via on the healing process of soft tissues in Wistar rats and also to compare it with the laser treatment. Wounds were produced in 60 Wistar rats. Two lesions were induced in each rat, one in the dorsal region and other in the hard palate, using circular surgical scalpels. The animals were divided into 3 groups: Group 1 (negative control) - treated with 0.9% physiological solution, Group 2 (positive control) - treated with laser, and Group 3 (experimental) - treated with vitamin B complex. The groups received daily treatments for 14 days. The lesion diameters were measured every three days. The lesions healing occurred in 14 days, showing that evolution of the animals treated with vitamin B complex was better during the treatment, when compared to control group, what may suggest that vitamin B complex influenced the soft tissue healing process (AU).

Evaluation of minimum inhibitory concentration and antimicrobial activity of basil extract ( Ocimum basilicum L. ) incorporated to the mouthwash

AIM: This study has as aim to evaluate the minimum inhibitory concentration and the antimicrobial activity of basil extract incorporated to the mouthwash against the bacteria S.mutans. MATERIAL AND METHODS: For the study, the hydro alcoholic basil extract (Ocimum basilicum L.) incorporated to the mouthwash was used in order to evaluate the effect of this formulation on bacteria and its Minimum Inhibitory Concentration (MIC). As positive control, TBS + S. mutans was used; as negative control, only the TBS; the fluid hydro alcoholic basil extract 20% and the concentrated basil extract 12%, both incorporated to the mouthwash, were also evaluated. As MIC verification method and antimicrobial activity, the micro dilution was used in the concentrations: pure, 1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128; and carried out in triplicate. The microtiter plates were incubated and evaluated after 24 and 72hs. RESULTS: The results showed there was no antimicrobial activity of mouthwash associated to the fluid and concentrated basil extract. However, the mouthwash insulated showed antimicrobial activity only as pure; other dilutions did not presented the same result. CONCLUSION: Before the findings in this study, it is possible conclude that hydro alcoholic basil extract incorporated to the mouthwash did not present antimicrobial activity against the bacteria S. mutans.